Histochemical methods for acid β-galactosidase: Technics for semipermeable membranes

Abstract

Indigogenic and azocoupling reactions for the detection of acid β-galctosidase in unfixed cold microtome sections adherent to semipermeable membranes are described. The indigogenic method is the method of choice. The described procedure prevents the leakage of the enzyme activity of sections (the diffusion is limited to the closest surroundings of the actual localization of enzyme activity) and is recommended as a routine method in studies concerning acid β-galactosidase.

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